In vitro Activity of Picroside I in Type 2 Diabetes Based on Oxidative Stress

Abstract. The primary factor leading to insulin resistance (IR) and type 2 diabetes mellitus (T2DM) is oxidative stress. Despite its liver-protecting, enzyme-lowering, immune-regulating, antiviral effects, the impact of picroside I on stress, glucose utilization, IR has not been investigated yet. In vitro studies were conducted evaluate antioxidant properties different concentrations I. results showed that effectively suppresses α-glucosidase α-amylase with IC50 values 109.75 μg/mL 160.71 in range 50-500 μg/mL. Additionally, when IR-HepG2 cells treated 80 I, it was found have little effect cell viability, increase consumption, decrease levels free radical metabolite malonic dialdehyde, superoxide dismutase activity. These findings indicate potential regulate stress cells, potentially improving exhibiting anti-T2DM Resumen. El principal que conduce a la resistencia insulina y tipo es el estrés oxidativo. A pesar de sus efectos protectores del hígado, reductores enzimas, inmunorreguladores antivirales, aún no se ha investigado impacto picrósido sobre oxidativo, utilización glucosa IR. Se realizaron estudios para evaluar las propiedades antioxidantes diferentes concentraciones Los resultados mostraron suprime eficazmente α-glucosidasa α-amilasa con valores 109,75 160,71 en rango 50 -500 microgramos/ml. Además, cuando células trataron encontró tenía poco efecto viabilidad celular, aumentaba consumo glucosa, disminuía los niveles metabolito radicales libres dialdehído malónico actividad superóxido dismutasa. Estos hallazgos indican tiene potencial regular oxidativo IR-HepG2, mejorando potencialmente exhibiendo anti-T2DM.